STRAIN OF BACTERIUM CORYNEBACTERIUM PSEUDOTUBERCULOSIS USED AS REFERENCE AND FOR PREPARING DIAGNOSTIC PREPARATIONS AT CAVERNOUS LYMPHADENITIS IN SHEEP

摘要

FIELD: biotechnology. SUBSTANCE: preparing strain C. pseudotuberculosis ВГНКИ N 70 R-ДЕП for identification acceleration of cavernous lymphadenitis pathogen in sheep at bacteriological, allergic and serological diagnosis of infection. Molecular properties of strain DNA: spectral indices: A 260/280 = 2.0, A 260/230 = 2.4, concentration is 1400 mcg/ml, melting point is 83.3 C, content of G + C = 71.8 mole%. On the basis of homology DNA nucleotide sequence strain is concerned to the group I. For diagnosis allergen prepared of this strain is administrated to sheep by intracutaneous route into subtail wrincle. Reaction recording is carried out after 24 hr. Inflammatory reaction appeared in sick animals at the place of diagnosticum administration. No inflammation in health animals. Method ensured to detect additionally up to 80% infected animals as compared with conventional method of diagnosis (by palpation of surface lymphatic nodes). EFFECT: accelerated identification of pathogen.