METHOD FOR PRODUCING MEDULLARY INHIBITOR OF HEMOPOIETIC TRUNCAL CELL PROLIFERATION

摘要

FIELD: medicine. SUBSTANCE: method involves obtaining and centrifuging medullary cells. The obtained cellular extraction is heated at 40-80 C during 10-60 min and dried before subjecting to centrifuging. Centrifuging is carried out at 3000-6000 RPM. The so produced extraction is cooled on ice, precipitated with acetone and solved precipitate is treated in chromatography column having reversed phase sorbent in system with triphluoracetic acid in acetonitryl gradient. EFFECT: improved output specific activity and homogeneity of end product.