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AG-F human T cell line with unique phenotype and cytokine secretions
AG-F human T cell line with unique phenotype and cytokine secretions
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机译:具有独特表型和细胞因子分泌的AG-F人T细胞系
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摘要
A human T cell line, designated AG-F, the cells of which: a) are capable of adherent growth as well as growth in suspension; b) have a short doubling time of approximately eight to nine hours; c) are near tetraploid, containing eighty-five to eighty-seven chromosomes instead of the usual forty-six; d) exhibit properties similar to those exhibited by T cells in very early stages of development; e) secrete a great variety of interleukins; and f) exhibit high CD4 antigen production, and the utilization of the AG-F cell line in hybridoma technology, including but not limited to lymphokine secretion studies, T cell lymphotropic virus studies, augmentation of antibody secretion in hybridomas and augmentation of cell growth and mitotic index by AG-F conditioned medium studies.
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