N- and C-terminal truncation and deletion mutants of human interleukin-3

摘要

Biologically active deletion and substitution mutants of hIL-3 are provided. Preferred mutants are those having one or more deletions at the N-terminus (amino acids 1-14) and/or the C-terminus (amino acids 116- 133, 120-130 and/or 130-133). Preferred substitution mutants include Cys. sup. 16 →Ala.sup.16 and/or Cys.sup.84 →Ala.sup.84, Glu.sup.50 . fwdarw.Lys.sup.50 and Lys.sup.79 →Glu.sup.79. These mutants can be used to formulate pharmaceutical compositions. Also disclosed are antibodies directed against specific epitopes localized between amino acids 29 and 54.