PAIR OF MINIRIBOZYME, MINIRIBOZYME-DIMER, CUTTING-DEACTIVATION OF TARGET RNA BY THEM AND MEDICINE

摘要

PROBLEM TO BE SOLVED: To obtain a new miniribozyme having a specific sequence, capable of cutting.deactivating an objective RNA when an NUX sequence exists in the other position in a connected region of the objective RNA even a 5'-NUX-3' sequence does not exist, and useful for diagnosis and curing, etc., of a disease relating to gene. ;SOLUTION: This pair of new miniribozymes is composed of a combination of a miniribozyme expressed by formula I {A is adenine monoribonucleotide; G is guanine monoribonucleotide; L is (3')-C-(5') (C is cytosine monoribonucleotide) or (3')-CN_p-(5') [N is A, G, C or U (U is uracyl monoribonucleotide); (p) is 1-10]; V is (3')-AGYAGUC-(5') (Y is A, G, C or U) or a single bond; Q is nucleic acid base complementary to a nucleic acid base of an objective RNA; (m) and (n) are each an integer} and a miniribozyme expressed by formula II [M is (3')-G-(5') or (3')-N_p'G-(5') (N' is a nucleic acid complementary to N); W is (3')-AAG-(5') or a single bond; R is a same kind as Q].;COPYRIGHT: (C)1997,JPO