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EXPRESSION CASSETTE CAPABLE OF IMPARTING MEDICINE METABOLIZING FUNCTION AND USE OF THE SAME

机译:可能影响药物代谢功能的表达盒及其使用

摘要

PROBLEM TO BE SOLVED: To obtain an expression cassette containing a promoter and terminator functioning in plant cells and a fused gene of a chlorophyll transferring signal peptide gene with a cytohrome p450 derived from a non plant body and capable of imparting a medicine metabolizing function to a plant. ;SOLUTION: This new expression cassette has (A) a promotor capable of functioning in plant cells, (B) a fused gene of a gene encoding a signal peptide for chlorophyll transfer expressed by the formula with a gene of a cytochrome P450 derived from a non plant body and (C) a terminator capable of functioning in plant cells, and can efficiently impart a medicine metabolizing function to a plant by introducing the same into the plant cells. The expression cassette is obtained by functionally joining 35S promoter derived from cauliflower mosaic virus as the promotor capable of functioning in the plant cells, and a NOS terminator derived from T-DNA, etc., as the terminator capable of functioning in the above fused gene and in the plant cells.;COPYRIGHT: (C)1997,JPO
机译:解决的问题:获得一种表达盒,其包含在植物细胞中起作用的启动子和终止子以及叶绿素传递信号肽基因与非植物体衍生的细胞色素p450的融合基因,并且能够赋予药物代谢功能。厂。 ;解决方案:此新的表达盒具有(A)能够在植物细胞中起作用的启动子,(B)编码由该分子式表达的叶绿素转移信号肽的基因的融合基因与来源于植物的细胞色素P450的基因融合非植物体和(C)能够在植物细胞中起作用的终止子,并且可以通过将其引入植物细胞中来有效地赋予植物药物代谢功能。通过将来自花椰菜花叶病毒的35S启动子作为能够在植物细胞中起作用的启动子和来自T-DNA等的NOS终止子作为能够在上述融合基因中起作用的终止子功能性连接,获得表达盒。 ;版权所有:(C)1997,日本特许厅

著录项

  • 公开/公告号JPH09252778A

    专利类型

  • 公开/公告日1997-09-30

    原文格式PDF

  • 申请/专利权人 SUMITOMO CHEM CO LTD;

    申请/专利号JP19960064330

  • 申请日1996-03-21

  • 分类号C12N15/09;A01H5/00;C12N1/21;C12N9/02;

  • 国家 JP

  • 入库时间 2022-08-22 03:34:24

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