METHOD FOR DISCRIMINATION OF NONHUMAN ANIMAL INFECTED WITH PARAHYDROPHOBIA VIRUS AND KIT FOR SAID METHOD

摘要

PROBLEM TO BE SOLVED: To identify an animal infected with Pseudo Rabies Virus(PRV) by subjecting a morbid animal to vaccination of PRV lacking glycoprotein gI or gp63. SOLUTION: pPR28 is consumed by PVuII and a cut piece, containing E.coli replication start point and bla gene, is recirculated to produce a plasmid comprising 4.9kbPvuII/BamH17 PVR fragment containing DNA arrangement for gI thus obtaining plasmid pPR28-1. BamH17 is subjected to subcloning, consumed, labeled and arranged. DNA arrangements for glycoprotein gp63 and gI are presented on arrangement tables (arrangement number 2 and 3). An animal infected actively with PRV can be detected using this DNA. Presence of PRV can be detected by wiping nose or throat using a cotton swab for the purpose of sampling and subjecting the sample to a standard hybridization of DNA/DNA.