MUTANASE-PRODUCING MICROORGANISM AND MUTANASE

摘要

PURPOSE: To obtain a new mutanase having excellent suppressing effect on the formation of dental plaque and sufficiently stably miscible to form a product of a composition for oral cavity application. ;CONSTITUTION: Bacillus sp. M7 strain (FERM P-14835) is cultured and the objective mutanase is collected from the culture product. The mutanase has high activity to decompose the α-1,3-glucoside bond of mutan and optimum working condition of pH4.5 and 50°C, stably keeps its activity at pH4-10 and ≤50°C and has a molecular weight of about 160,000 by SDS-polyacrylamide gel electrophoresis. The activity of the enzyme is inhibited by mercury, silver and p-chloromercury benzoic acid. The enzyme exhibits extremely excellent plaque-removing effect even by the single use.;COPYRIGHT: (C)1996,JPO