NEW NITRILASE GANE

摘要

PROBLEM TO BE SOLVED: To obtain the subject new gene that is capable of increasing outstandingly the catalytic ability of microorganisms to hydrolyze a nitrile compound by gane recombination technique for a gane that codes for polypeptide having nitrilase activity and a specific amino acid sequence. ;SOLUTION: This nitrilase genetic DNA is a new genetic DNA that has an amino acid sequence expressed by the formula (Xaa is a deletion or Met) and codes for polypeptides having nitrilase activity, and includes analogues of the genetic DNA which are derived from homonymity of a genetic code. Because this nitrilase gene enables many copies of the nitrilase gene cloned by a method of genetic recombination to exist in bodies of microorganisms, it can increase the catalytic ability of microorganisms to hydrolyze nitrile compounds. This genetic DNA is obtained by culturing Gordona terrae MA-1 stain (FERM BP-4535), separating its chromosome DNA by a conventional method to prepare its DNA library, and by screening the library by using a probe consisting of part of the nitrilase gene.;COPYRIGHT: (C)1997,JPO