Isolated refinement manner and soybean saponin null of soybean

摘要

PURPOSE:To obtain a new means for isolating and purifying soybean saponin as a true saponin and the soybean true saponin isolated thereby. CONSTITUTION:A soybean glycoside ingredient is extracted from soybean as a raw material and the resultant extracted solution is directly adsorbed in an octadecylsilyl(ODS) column and then eluted with a hydrous methanol. The eluted ingredient is subsequently analyzed by thin-layer and liquid chromatographies. Respective eluted soybean saponin fractions selected therefrom are separately collected, concentrated and evaporated to dryness. Thereby, the objective soybean saponin substance is isolated.