Is induced with the ethylene of the azuki bean acidic kichinaze

摘要

PURPOSE: To obtain the title enzyme usable for breeding plants having high disease and insect pests and pathogenic germ resistances, having ability of hydrolyzing polysaccharides bonded with N-acetyl-D-glucosamine by β-1,4 bond isolated from a primary leaf of azuki bean. ;CONSTITUTION: A primary leaf of azuki bean treated with ethylene is cut off, treated with 20ppm ethylene for 24 hours, a protein is extracted with a buffer solution of acetic acid having pH4.0 to give a crude extracted solution, which is subjected to ammonium sulfate salting out and then to anion exchange chromatography to collect a chitinase active fraction. The fraction is subjected to gel filtration and a chitinase fraction eluted at 21,000 molecular weight is recovered to give the objective protein which has an amino acid sequence deficient in an amino acid sequence from N end to 34 amino acid sequences among an amino acid sequence of the formula and chitinase activity of acting polysaccharides prepared by polymerizing N-acetyl-D-glucosamine by β-(1,4) bond, hydrolyzing the bond and libreating N-acetyl-D-glucosamine.;COPYRIGHT: (C)1993,JPO&Japio