Identification, cloning and use of an amplified DNA region in strains of P. chrysogenum which are superproducers of penicillin

摘要

Identification, cloning and use of an amplified DNA region in strains of P. Chrysogenum which are superproducers of penicillin. The said amplified region has a size of 57 kb in P. Chrysogenum E-1 and 106 kb in P. chrysogenum AS-P-78 and includes the penicillin biosynthesis genes pcbAB(-(L--aminoadipyl)-L- cisteinyl-D-valine synthetase), pcbC (isopenicillin-N synthase) and penDE (acyl-CoA:6-APA acyltransferase), which extend over 16.5 kb. A direct relationship has also been established between the number of copies of this region and penicillin production capacity. A series of applications are also presented with a view to increasing the number of copies of this region in a specific strain and consequently increasing their penicillin production.