Enzyme kinetics assay to determine the CO2 content of body fluids using phosphoenolpyruvate carboxylase inhibitor.

摘要

KINETIC TEST DESCRIBED AN IMPROVED TO DETERMINE ESPETROFOTOMETRICAMENTE CARBON DIOXIDE (CO SUB 2) dissolved in the body fluid where in a PH 8.0, the SUB CO 2 is present substantially as bicarbonate ion (HCO SUB 3). SAMPLE, undergoes a reaction mixture containing phosphoenolpyruvate (PEP) carboxylase and FOSFOENOLPIRUVATA (PEPC) catalyzes enzymatically THE CONVERSION OF SUB HCO 3 A oxaloacetate (OA). OA produced is subjected to a second reaction mixture containing NADH DEHYDROGENASE AND MALATA (MDH) reducing OA to malate and oxidizes NADH to NAD +. SUB LEVEL CO 2 in the sample can be indirectly determined by measuring the change in concentration of NADH. ADDING AN INHIBITOR TO reaction mixture RETURNS THE KINETIC REACTION SYSTEM ESSENTIALLY FIRST ORDER ALL SERIES ON INTEREST bicarbonate concentration. They HAVE THE BEST RESULTS OBTAINED THROUGH THE USE OF AN INHIBITOR PROVIDING A SELECTED Anion group consisting of C10 SUB 3 SUB C10 4 SCN, O I.