RECOMBINANT PROTEINS HAVING MULTIPLE DISULFIDE BONDS AND THIOL-SUBSTITUTED CONJUGATES THEREOF

摘要

The present invention relates to recombinant antigen binding proteins havingmultiple disulfide bonds useful for the preparation of immunoconjugates. Inparticular, this invention relates to recombinant antibodies comprising anIgG3 hinge region and lacking a CH2 constant domain. These mutated antibodiesare used to bind a diagnostic or therapeutic agent through one or more reduceddisulfide bonds in the antibody hinge region. Thus, the invention contemplatesthe use of such immunoconjugates in diagnosis and therapy.