Recombinant proteins having multiple disulfide bonds and thiol-substituted conjugates thereof

摘要

The present invention relates to recombinant antigen binding proteins having multiple disulfide bonds useful for the preparation of immunoconjugates. In particular, this invention relates to recombinant antibodies comprising an IgG3 hinge region and lacking a CH2 constant domain. These mutated antibodies are used to bind a diagnostic or therapeutic agent through one or more reduced disulfide bonds in the antibody hinge region. Thus, the invention contemplates the use of such immunoconjugates in diagnosis and therapy.