INTEGRATED NUCLEIC ACID HYBRIDIZATION DEVICES BASED UPON ACTIVE SURFACE CHEMISTRY

摘要

The hybrization device is comprised of an oligonucleotide probe and a solidsubstrate wherein the solid substrate has a support surface with a neutral ornegative electrostatic field and a hybridization surface. As shown in thefigure, the hybridization surface is accessible for linking theoligonucleotide probe to the solid substrate. The oligonucleotide probe islinked to the hybridization surface of the solid substrate at a distance of nomore than 100 angstroms. Further, there is a method of using the hybridizationdevice to detect single base changes in DNA or RNA target sequences.