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A NOVEL FUNGAL MULTISUBUNIT PROTEIN COMPLEX CRITICAL FOR EXPRESSION OF FUNGAL PROTEINS
A NOVEL FUNGAL MULTISUBUNIT PROTEIN COMPLEX CRITICAL FOR EXPRESSION OF FUNGAL PROTEINS
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机译:表达真菌蛋白的新型真菌多亚基蛋白复合物至关重要
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摘要
The present invention provides a complex of fungal polypeptides, termed TAFs, that are necessary for activated transcription in fungi such as S. cerevisiae. The complex comprises at least nine associated subunit polypeptides, having molecular masses of about 180 kDa, 145 kDa, 116 kDa, 90 kDa, 68 kDa, 51-54 kDa, 47 kDa, and 30 kDa, respectively. TAF-145, having the sequence set forth in FIG. 6B, binds TATA-box Binding Protein (TBP). The invention also includes nucleic acid sequences encoding TAF-145, as well as DNA vectors and transformed cells suitable for recombinant expression of this polypeptide.
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