Biosensor for simultaneous measurement of glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase enzyme activity and method for producing the same

摘要

The present invention relates to a biosensor capable of simultaneously measuring glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase using only one enzyme of clutamate dehydrogenase.;The biosensor of the present invention includes a step of forming a reference electrode on an insulating substrate, a step of forming first and second indicating electrodes on a substrate on one side of the reference electrode, Forming third and fourth indicating electrodes so as to face the first and second indicating electrodes, forming a carbonaceous layer on the first, second, third, and fourth indicating electrodes, , Forming an enzyme / polymer carbonaceous layer on the carbon paste on the second indicating electrode, forming an enzyme / polymer carbonaceous layer on the carbon paste on the third and fourth indicating electrodes, Polymeric carbon paste layer formed on the first indicating electrode and the non-enzyme / polymeric carbon paste layer formed on the third indicating electrode, and forming an amino acid / polymer paste layer for the GPT enzyme reaction on the third indicating electrode, / Polymer And a step of forming an amino acid / polymer paste layer for GOT enzyme reaction on the non-enzyme / polymer carbon paste layer formed on the carbon paste layer and the fourth indicating electrode.