A method for separating active ingredients of kusu-ku, and its pharmacological activity

摘要

The present invention relates to a method for separating a component having pharmacological activity from a fresh picked herb and a use thereof, and a method for separating an active component from a fresh pickle, and a method for identifying the pharmacological action of these components, To provide the use of the active ingredient.;In the present invention, the shiitake mushroom ground part is shredded after shrimp, and the mixture is refluxed in a water bath to extract methanol several times, and the solvent is removed under reduced pressure to obtain a methanol extract. This methanol extract was then fractionated and subjected to silica gel column chromatography to obtain luteolin-7-O--D-glucoside and luteolin-7-O-Remove luteolin-7-O-rutinoside.;The methanol extract and luteolin-7-O--D-glucoside thus separated decreased the total cholesterol and total lipid in the fat tissue weight of blood in hyperlipidemia induced by high fat diet,