Method for producing the vacuolar cytotoxin protein of Helicobacter pylori from recombinant E. coli

摘要

The present invention relates to a method for producing a vacuolar cytotoxin (Vac), which is an antigenic determinant protein of Helicobacter pylori (H. pylori), in the form of a fusion protein using E. coli, Escherichia coli transformed with a Ub-V200 fusion protein expression vector containing a fusion gene in which a ubiquitin gene linked to a gene encoding a protein (V200) consisting of the 168th amino acid to the 367th amino acid of Laurie Vac is subjected to conditions suitable for the expression of the fusion protein , Pulverizing the recombinant E. coli cells expressing the fusion protein, washing the insoluble precipitate with a buffer solution containing triton, dissolving with urea, and performing gel filtration chromatography and heparin-sepharose chromatography Of H. pylori infection according to the method of producing the Ub-V200 fusion protein of the present invention The Ub-V200 fusion protein that can be useful for vaccine development can be mass-produced with high yield.