2. pcbC gene producing 3-dihydroxybiphenyl dioxygenase

摘要

Pseudomonas sp. The 2, 3-dihydroxybiphenyl dioxygenase isolated from the P20 strain is an enzyme that decomposes 4-chlorobiphenyl, which is a poorly degradable contaminant of aromatics. The nucleotide sequence of the pcbC gene encoding it is determined and isolated from other strains An alternative system for the growth of chlorinated biphenyls to be converted to chlorinated benzoates was observed as compared to the gene for production of 2, 3-dihydroxybiphenyl dioxygenase. The PcbC pollen produced by encoding the pcbC gene The amino acid sequence of the peptide was analyzed and it was found that it plays an important role in the activity of the fixed Gly-His 2, 3-dihydroxybiphenyl dioxygenase existing in the active site by comparing the similarity with other strains.