ATTENUATED COLD-ADAPTED INFLUENZA VIRUS STRAIN USED FOR PREPARING LIVE INTRANASAL INFLUENZA VACCINE

摘要

FIELD: medicinal virology. SUBSTANCE: vaccine strain GKV N 2309 is obtained after 60 passages of virus B/USSR/69 at 25 C. Strain shows the enhanced reproductivity in developing chicken embryos at 25 C in 1 l ЭИД 50/0.2 ml, 4.0 lg, i. e. its is cold-adapted. Hemagglutinating activity of strain: 1:64-1:256; infectious activity: 6.0-7.5 lg ЭИД 50/0.2 ml (infectious embryonal doses). Strain does not change properties after 5 passages in chicken embryos at optimal temperature, strain is reactive and immunogenic for adult and children. Strain can be used for live influenza vaccine production for influenza prophylaxis in adult and children. EFFECT: improved method of vaccine preparing, strain indicated above.