Coumarin-labeled nucleoside 5'-triphosphates

摘要

A fluorescent label compound having the formulaP PA--B. sup.1 --B.sup.2 --B.sup.3 --CPPwhereinP PA represents the residue of a natural or synthetic nucleotide or nucleoside or a derivative thereof;PPB.sup.1 represents a divalent spacer radical or a single bond;PPB.sup.2 represents a divalent spacer radical;PPB.sup.3 represents a divalent spacer radical or a single bond; andPPC represents a coumarin radical having the formula ##STR1## in which R.sup. 1 represents hydrogen or cyano;PPR.sup.2 represents phenyl or thiazolyl bonded in the 2-, 4- or 5-position, wherein the phenyl is unsubstituted or substituted by nitro, cyano, amino, --NH--C. sub.1-4 - alkyl, --(CH.sub.2).sub.1-4 --NH.sub.2, --(CH.sub.2).sub.1-4 -- NH--(CH. sub.2).sub.1-3 --CH.sub.3, carboxy, C.sub.1-4 -alkoxycarbonyl, C. sub.1-4 - alkoxycarbonyloxy, hydroxy, C.sub.1-4 -alkylaminocarbonyl, or C. sub.1-4 - alkylcarbonylamino and either further unsubstituted or substituted by C. sub.1-4 -alkyl, fluorine, chlorine or bromine, and where the thiazolyl is unsubstituted or monosubstituted or disubstituted by chlorine, cyano, carboxyl, or C.sub.1-4 -alkoxycarbonyl, or the thiazolyl is fused in the 4- and 5-position with a benzene ring which is either unsubstituted or substituted by carboxyl, amino, or hydroxyl; PPR.sup.3 represents hydrogen, C.sub.1-4 -alkyl, C.sub.1-4 - alkoxycarbonyl-C.sub.1- 4 -alkyl, or phenylsulphonyl, where the C.sub.1-4 -alkyl is unsubstituted or substituted by hydroxyl, amino, carboxyl, or C. sub.1-4 -alkoxycarbonyl, and where the phenylsulphonyl is unsubstituted or monosubstituted or disubstituted by chlorine, bromine, or C.sub.1-4 - alkyl;PP or where one of the radicals R.sup.2 or R.sup.3 denotes or is substituted by a primary or secondary amino group, hydroxyl, carboxyl, or C.sub.1-4 - alkoxycarbonyl, or can be converted into such a group by hydrolysis or hydrogenation.