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High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
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机译:使用互补寡核苷酸的洗脱和测序对核酸序列或遗传变异进行高通量筛选的方法
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摘要
The present invention pertains to high-throughput screening methods to identify genetic alterations in DNA samples. A multiplicity of DNA samples are immobilized on a solid support and hybridized simultaneously with a mixture of oligonucleotides representing variant sequences. Hybridizing oligonucleotides are then eluted from each DNA sample individually and their sequence is determined. The methods of the present invention allow the identification of disease-causing mutations and polymorphisms in patients' DNA, as well as the identification of disease- causing microorganisms.
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