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Hematopoietic stem cell growth-promoting compositions containing a fibroblast-derived fragment of fibronectin and a growth factor, and methods employing them in vitro or in vivo
Hematopoietic stem cell growth-promoting compositions containing a fibroblast-derived fragment of fibronectin and a growth factor, and methods employing them in vitro or in vivo
An 18 kDa protein, denominated FDF-3, has been isolated from cultures of human fibroblasts and has been found to potentiate the growth- and differentiation-promoting activities of certain cytokines toward hematopoietic progenitor cells. The amino acid sequence of the FDF-3 protein, shown in SEQ ID NO: 14, corresponds to the sequence of a C- terminal domain of human fibronectin. The protein is useful in combination with IL-3, G-CSF, GM-CSF, or SCF (stem cell factor) in the expansion of hematopoietic cell populations in vitro or in therapeutic methods to promote recovery from leukopenia or myelosuppresion in vivo.
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机译:从人类成纤维细胞的培养物中分离出一种18 kDa的蛋白,命名为FDF-3,发现该蛋白能增强某些细胞因子对造血祖细胞的生长和分化促进活性。 SEQ ID NO:14所示的FDF-3蛋白的氨基酸序列对应于人纤连蛋白的C端结构域的序列。该蛋白可与IL-3,G-CSF,GM-CSF或SCF(干细胞因子)结合使用,在体外扩增造血细胞群或在治疗方法中促进体内白细胞减少或骨髓抑制的恢复。
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