3H1,2 benzo dithiol 31,1 dioxide was used, - synthesis of the upper 35S indicator oligonucleotide

摘要

(57) Abstract This invention offers the new chemical compound of ³⁵ S indicator oligonucleotides. This chemical compound is ³⁵ S3H1,2 benzo dithiol 3 on 1,1 dioxides (1). With constitutional formula (1), the asterisk shows the position of ³⁵ S. Furthermore, it is something which also the manner which synthesizes this chemical compound is offered, ³⁵ S thiobenzoic acids (4) thio salicylic acid (5) it makes contact first in the Acid solvent, you obtain condensate ³⁵ S3H1,2 benzo dithiol 3 on (2) it consists of the fact that. After that, making oxidize ³⁵ S3H1,2 benzo dithiol 3 on (2), with the appropriate oxidizer like trifluoro acetic acid and hydrogen peroxide, the product ³⁵ S3H1,2 benzo dithiol 3 on 1,1 dioxide of desire you obtain (1). With 3H1,2 benzo dithiol 3 on 1,1 dioxide it is possible with ³⁵ S3H1,2 benzo dithiol 3 on 1,1 dioxides (1) oxidation as for all oligonucleotides which are easy xanthation, the indicator to be done. Therefore, this invention the oligonucleotide offers also the new manner which ³⁵ S indicators is done. This chemical compound and manner although bio distribution and resolution of anchisensuorigonukureochido are traced at the time of pharmacy dynamic researching are useful.