Human fetal lung fibroblast diploid cell line useful for virus production and method for producing varicella-zoster virus using the same

摘要

PCT No. PCT/KR97/00028 Sec. 371 Date Oct. 14, 1997 Sec. 102(e) Date Oct. 14, 1997 PCT Filed Feb. 15, 1997 PCT Pub. No. WO97/30147 PCT Pub. Date Aug. 21, 1997A human embryonic lung fibroblast diploid cell strain LBHEL(KCTC 0127BP) is highly susceptible to varicella zoster virus(VZV). A cell-free varicella zoster virus (VZV) is produced by (a) culturing the human embryonic lung fibroblast diploid cell strain LBHEL(KCTC 0127BP) of claim 1 in a culture vessel to give cultured LBHEL cells; (b) infecting the cultured LBHEL cells with VZV to give VZV-infected cells; (c) culturing and harvesting the VZV-infected cells; (d) disrupting the harvested cells to obtain a cell homogenate; and (e) centrifuging the cell homogenate to obtain a supernatant containing the cell-free VZV.