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How to increase the stearate content of vegetable seed oils and plant stearoyl -ACP thioesterase array

机译:如何增加植物种子油和植物硬脂酰-ACP硫酯酶阵列的硬脂酸酯含量

摘要

(57) plant thioesterase, [summary] The present invention relates to plant acyl -ACP thioesterase having significant activity against stearoyl -ACP especially. I describe DNA constructs useful for the expression in plant seed cells in the plant stearoyl -ACP thioesterase. Such constructs, control of regulatory elements capable of directing the expression of plant stearoyl -ACP thioesterase preferentially in seed tissues as compared to other plant tissues when expressed in transgenic plants of this I containing a DNA sequence encoding a plant stearoyl -ACP thioesterase for the purpose under. The present invention also relates to a method for modifying the stearic acid produced in plant seed cells using a DNA sequence encoding a plant thioesterase stearoyl -ACP. Plant thioesterase with significant activity against stearoyl -ACP exemplified herein is derived from the mangosteen. Stearic acid concentration as modified, may be obtained by combination with an antisense construct reduces the stearoyl -ACP desaturase or natural, alone mangosteen thioesterase, and expressed in plant seeds.
机译:(57)植物硫酯酶,总的来说,本发明涉及尤其对硬脂酰-ACP具有显着活性的植物酰基-ACP硫酯酶。我描述了可用于在植物硬脂酰-ACP硫酯酶中在植物种子细胞中表达的DNA构建体。当在此I的转基因植物中表达时,这样的构建体控制能够指导种子中植物硬脂酰-ACP硫酯酶的表达优先于其他植物组织的调节元件的调控,该目的包含编码植物硬脂酰-ACP硫酯酶的DNA序列。下。本发明还涉及使用编码植物硫酯酶硬脂酰-ACP的DNA序列修饰植物种子细胞中产生的硬脂酸的方法。本文所例示的对硬脂酰-ACP具有显着活性的植物硫酯酶衍生自山竹果。修饰的硬脂酸浓度可通过与反义构建体组合使用来降低硬脂酰-ACP去饱和酶或天然的单独山竹果糖硫酯酶的表达,并在植物种子中表达。

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