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LOW-TEMPERATURE ALKALI PROTEASE T16, MICROORGANISM CAPABLE OF PRODUCING THE SAME, PRODUCTION OF THE SAME, AND DETERGENT COMPOSITION CONTAINING THE ENZYME AND ENZYME PREPARATION FOR FOOD PROCESSING
LOW-TEMPERATURE ALKALI PROTEASE T16, MICROORGANISM CAPABLE OF PRODUCING THE SAME, PRODUCTION OF THE SAME, AND DETERGENT COMPOSITION CONTAINING THE ENZYME AND ENZYME PREPARATION FOR FOOD PROCESSING
PROBLEM TO BE SOLVED: To obtain the subject new enzyme, capable of hydrolyzing casein, also acting on hemoglobin or keratin, and useful for detergents, cosmetics, food modification, as a digestive auxiliary, antiinflammatory agent, etc., by culturing Sphingomonas sp. KSM-T16. ;SOLUTION: This enzyme, a new low-temperature alkali protease T16, is obtained by culturing Sphingomonas sp. KSM-T16 (FERM P-15457) and collecting the product, being useful for detergents, cosmetics, food modification, as a beer clarifier, digestive auxiliary, antiinflammatory agent, etc. This new enzyme has the following characteristics: acting at least 0-60°C; optimum temperature is 28-32°C; acting at pH5-12; optimum pH is about 9; capable of thoroughly hydrolyzing casein; also acting on hemoglobin, keratin; apparent estimated molecular weight determined by gel chromatography using Sephacryl S-200 is 26,000±1,000; and subject to inhibition by Hg2+, ethylenediaminetetraacetic acid, phenylmethanesulfonyl fluoride, etc.;COPYRIGHT: (C)1997,JPO
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