LOW-TEMPERATURE ALKALI PROTEASE T15, MICROORGANISM CAPABLE OF PRODUCING THE SAME, PRODUCTION OF THE SAME, AND DETERGENT COMPOSITION CONTAINING THE ENZYME AND ENZYME PREPARATION FOR FOOD PROCESSING

摘要

PROBLEM TO BE SOLVED: To obtain the subject new enzyme, capable of thoroughly hydrolyzing casein, also acting on hemoglobin or keratin, and useful for detergents, cosmetics, food modification, as a digestive auxiliary, antiinflammatory agent, etc., by culturing Bacillus sp. KSM-T15. ;SOLUTION: This enzyme, a new low-temperature alkali protease T15, is obtained by culturing Bacillus sp. KSM-T15 (FERM P-15456) and collecting the product, being useful for detergent compositions, enzyme preparations for food processing, etc. This new enzyme has the following characteristics: acting at least 0-60°C; optimum temperature is 28-32°C; acting at pH5-12; optimum pH is about 11; capable of thoroughly hydrolyzing casein; also acting on hemoglobin, keratin; estimated molecular weight determined by sodium dodecylsulfate- polyacrylamide gel electrophoresis is 37,000±1,000; and subject to inhibition by Hg²⁺, ethylenediaminetetraacetic acid, phenylmethanesulfonyl fluoride, chymostatin, etc.;COPYRIGHT: (C)1997,JPO