NUCLEIC ACID CODING ENZYME PARTICIPATING TO BIOSYNTHESIS OF OMEGA-CYCLOHEXANE FATTY ACID, NUCLEIC ACID PRIMER CONTAINING PART OR WHOLE OF BASE SEQUENCE OF THE NUCLEIC ACID, AND DETECTION AND IDENTIFICATION OF MICROORGANISM BELONGING TO GENUS ALICYCLOBACILLUS

摘要

PROBLEM TO BE SOLVED: To quickly and easily perform detection and identification of a heat- resistant acid-fast bacterium propagating in a fruit juice (or a drink containing the juice) by a primer synthesized from a base sequence of a gene coding an enzyme participating to biosynthesis of an -cyclohexane fatty acid specific to the heat-resistant acid-that bacterium. ;SOLUTION: Presence of a microorganism propagating in a specimen is certified by obtaining a gene derived from a microorganism belonging to a heat-resistant acid-fast bacterium (preferably, a microorganism belonging to genus Alicyclobacillus, especially Alicyclobcillus acidocaldarius) and coding an enzyme participating in biosynthesis of an -cyclohexane fatty acid, synthesizing a primer according to its base sequence and judging whether a DNA existing in the specimen is amplified by the nucleic acid primer or not.;COPYRIGHT: (C)1998,JPO