One-dimensional distribution fractionation method

摘要

A method for demarcating a reticulocyte particle group from a red blood cell particle group in a one-dimensional frequency distribution assumes a normal distribution on one side of the distribution. Various positions Xs1, Xs2, . . . , Xsn, which respectively indicate frequencies that are p1, p2, . . . pn percent (where n is an integer of 3 or greater) of a peak frequency of the distribution, on the normal side of the distribution, are determined. An average value and standard deviation of the normal distribution is determined from these values. A threshold value is next obtained from the average value and standard deviation, the threshold value being used to separate the reticulocyte group from the red blood cell group. The average value and standard deviation may be obtained using a least square method or by computing the mean of several average values and standard deviations.