Method of detection null of the mutation gene of the nuclear chromosome which works in mitochondria homologous

摘要

PURPOSE: To detect a recessive variant gene useful for diagnosis, etc., of mitochondrial diseases in variant genes of a nuclear chromosome acting on mitochondrial homologous recombination. ;CONSTITUTION: The cell fusion of a nucleated cell containing a mitochondria having a marker gene (I) and a nonnucleated cell to a mitochondria having a marker gene (II) different from the marker gene (I) is carried out and a fused cell having a recombinant type mitochondria having the marker gene (I) and the marker gene (II) in the resultant fused cell is selected to thereby detect the presence or absence of a variant gene of a nuclear chromosome acting on the mitochondrial homologous recombination from a reduction in incident frequency of the fused cell.;COPYRIGHT: (C)1997,JPO