Production manner null of natural die abushijin

摘要

PURPOSE:To obtain the title abscisic acid in high efficiency by putting fungi classified as Botrytis to culture using a liquid medium containing a hot water liquid extract from citrus fruits skin to effect production and accumulation of natural-type abscisic acid which is then isolated and collected from the resultant culture solution. CONSTITUTION:Citrus fruits skin is immersed in hot water at 50-100 deg.C for ca.30min followed by filtration or decantation to produce a liquid extract. This extract is then added to a liquid culture medium containing (1) a carbon source such as bran, (2) a nitrogen source such as defatted soybean powder and (3) a salt such as magnesium salt, and fungi such as Botrytis cinerea FERM P-6165 classified as Botrytis is inoculated into the culture medium followed by making a culture at 10-40 deg.C. After the culture, the fungi is removed from the resultant culture solution by centrifugation and the supernatant thus produced is allowed to absorb on activated carbon followed by dissolving out natural-type abscisic acid with an organic solvent and isolating and purifying said acid. Thereby the objective natural-type abscisic acid can be obtained through high-level accumulation at a high fermentation rate, thus enabling this acid to be produced on an industrial scale.