首页> 外国专利> BOX OF ANALYSIS FOR DETERMINING THE LEVEL OF THE INDEX GERMINATION BACTERIA IN WHEY.

BOX OF ANALYSIS FOR DETERMINING THE LEVEL OF THE INDEX GERMINATION BACTERIA IN WHEY.

机译:确定乳清指数萌发细菌水平的分析框。

摘要

The present invention relates to an analysis kit for a method of determining the level of bacterial germination index in the whey, wherein the whey sample is diluted with a reagent solution containing 4 ethoxylate - (1,1,3,3-tetramethylbutyl) phenol, the diluted sample is centrifugally separated into a liquid phase and a solid residue, a reactive solution containing a bacteriolytic reagent is added to the solid residue to convert it into suspension, the suspension is mixed with a reagent solution hydrolyzing adenosine triphosphate to obtain a reaction mixture is left to react the reaction mixture for a time sufficient to effect hydrolysis of adenosine triphosphate, bacterial triphosphate is released adénosie contained in the reaction mixture by treatment with a reactive solution releasing bacterial adénosie triphosphate was added to the reaction mixture a reactive solution of luciferin-luciferase which buffers around a pH of 7.0 to 7.9.
机译:本发明涉及一种用于确定乳清中细菌发芽指数水平的方法的分析试剂盒,其中所述乳清样品用含有4-乙氧基化物-(1,1,3,3-四甲基丁基)苯酚的试剂溶液稀释,将稀释后的样品离心分离成液相和固体残留物,将包含杀菌剂的反应溶液加入到固体残留物中以将其转化为悬浮液,将悬浮液与水解三磷酸腺苷的试剂溶液混合以获得反应混合物。使反应混合物反应足以使三磷酸腺苷水解的时间,通过用反应溶液处理释放反应混合物中所含的细菌三磷酸腺苷,从而将细菌三磷酸腺苷添加到反应混合物中,将荧光素-萤光素酶可在7.0至7.9的pH值附近缓冲。

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