METHOD FOR THE PREPARATION OF CELLULAR CULTURAL INACTIVATED VACCINE AGAINST FOWL PEST

摘要

The vaccine is inactivated by ethanol and is harmless. Theantigene for its preparation is produced after cultivation underroller conditions of the local cellular cultural strain DD/94 ofcells of the permanent line VIC21. The viral suspension producedwith infection titre 107.5 KID50 is adsorbed in 2% gel ofaliminium hydroxide and is settling for 48 h, after which thesupernatant is removed to 50% of the initial volume and isinactivated by ethanol. As an oleoadjuvant to the antigene mineraloil emulsified by montanide 888 is added in 3:2 ratio. Theimmunogeneity of the vaccine is tested on 18-20-week chicksimmunized with live vaccine in advance. On the 25th day after thevaccination a group of 10 chicks are infested by a virulent strainof the fowl pest virus. Simultaneoulsy a control group of 10unvaccined chicks is also infested. For 15 days 90% of thevaccined chicks remain in good health, and 90% of the controls diewith symptoms of fowl pest.2 claims.