Hybridization probe is for diagnosing the method that vein border strawberry virus prepares it

摘要

The vein border diagnosis strawberry virus of hybridization probe is by stock DNA (DNA), its sequence is complementary to DNA sequence dna vein border strawberry virus and gives in Table I, or to the length of its arbitrary section be at least 12 bases (preferably 20 to 600 bases), and it is located at any nucleotide containing at least one radioactivity or non-radioactive marker. The preparation of given isolated DNA probe, make strawberry vein border disease virus or its any segment, then with the nucleic acid or its analog of the triphosphate base of denaturation mixtures incubated, advantageously deoxyadenosine triphosphate, deoxyguanosine triphosphate, deoxycytosine triphosphates and deoxythymidine triphosphate, random oligonucleotide, advantageously hexanucleotides or nucleotide contain non-radioactive marker in the presence of polymerase, advantageously archaeal dna polymerase, or the nucleotide advantage radioactively modified is gamma amino butyric acid P32atriphos in the presence of kinase enzyme, the advantages of with T4 polynucleotide kinases, in the presence of kinase enzyme S35atriphos, the advantages of T4 polynucleotide kinases.