Method for obtaining pyruvate decarboxylase acyloin prepares this pyruvate decarboxylase, the method that the corresponding coding PDC-gene of DNA sequence dna obtains acyloin

摘要

The present invention relates to pyruvate decarboxylases( PDC )Conversion pyruvic acid in the presence of to(R values)Benzaldehyde-Phenylacetylcarbinol(Ⅰ)Product enantiomeric purity ≥95% and product ratio I to 2-hydroxypropiophenone ≥95% and specific activity be to form product I reduction ratios 1U/mg,Whose trp residue position 392 is by the natural fat race amino acid of free radical,It is further processed to obtain( PDC )It is greater than 1U/mg with the specific activity of pyruvate decarboxylase and is used to form Phenylacetylcarbinol,By the way that it is isolated from production organism,With coding PDC from Zymomonas motion fermentation,The 1174-1176 bit codon TGG codes for amino acid tryptophan resi-dues 392 of its DNA sequence dna are by the natural fat race amino acid of codon coding free radical. 392 also required in enzymatic preparation acyloin by acid in enzymatic acyloin condensation { alpha }-ketocarbonic and/or aldehyde in the presence of pyruvate decarboxylase (PDC) whose trp residue in the present invention replace the natural fat race amino acid for having free radical.