PROCESS FOR THE ENZYMATIC CENSUS OF RECOMBINANT PROTEINS WITH IGA-PROTEASES AND OBTAINING OF FUNDED PROTEIN, AND PHARMACEUTICAL COMPOSITION

摘要

For the enzymatic cleavage of fused proteins and obtaining of desired portions of these fused proteins, (1) genetic engineering means are used to modify a junction region in which two portions of the fused protein are connected together so that in this junction region at least one IgA protease recognition site with the amino-acid sequence Y-Pro. &darr& .X-Pro is produced, where X can be any amino acid and Y can be any one or more amino acids, (2) the fused protein resulting from step (1) is cleaved by IgA protease at the position identified by . &darr& . in the recognition site and (3) after the cleavage one or more desired portions of the fused protein are obtained.