Isolation and Purification of Enzymatic Hydrolysates of Yam （Dioscorea opposita Thunb. ） Proteins

摘要

Using yam( Dioscorea opposita Thunb.) as the experimental material,enzymatic hydrolysates of yam proteins were prepared with alkaline protease,which were then isolated and purified by cellulose DE-52 anion exchange chromatography Sephadex G-50 gel chromatography. According to the results,four absorption peaks were obtained by cellulose DE-52 anion exchange chromatography,and fractionsat each absorption peak were collected. Specifically,the reducing ability of four peaks demonstrated a descending order of peak 2( P2) > peak 1( P1) > peak 3( P3) > peak 4( P4). By Sephadex G-50 gel chromatography,P1 and P2 were isolated and purified with distilled water and Tris-HCl buffer,and two absorption peaks were obtained,respectively.