IDENTIFICATION OF A TRANSFORMING FRAGMENT OF HERPES SIMPLEX TYPE 2 AND DETECTION THEREOF IN CLINICAL SPECIMENS

摘要

The present invention relates to oligonucleotide probes derived from HSV-2, capable of selectively hybridizing thereto and to a subsequence of HSV-2 Bgl II N from which the oligonucleotide probes were derived. Further, the invention relates to an optimized assay of nucleic acid amplification permitting the sensitive and specific detection in clinical speciments of HSV2 as well as a specific typing of the HSV in the sample. The present invention further relates to kits for the detection and typing of the HSV in a sample. In addition, the invention provides the nucleic acid and amino acid sequence of a subsequence of HSV-2 Bgl II N having transforming activity. Further, the invention teaches diagnostic and therapeutic methods for genital cancer comprising the use of these sequences or ligands directed thereto.