IDENTIFICATION OF A TRANSFORMING FRAGMENT OF HERPES SIMPLEX TYPE 2 AND DETECTION THEREOF IN CLINICAL SPECIMENS

摘要

The present invention relates to oligonucleotide probes derived from HSV-2,capable of selectively hybridizing thereto and to asubsequence of HSV-2 Bgl II N from which the oligonucleotide probes werederived. Further, the invention relates to an optimized assayof nucleic acid amplification permitting the sensitive and specific detectionin clinical speciments of HSV-2 as well as a specific typing ofthe HSV in the sample. The present invention further relates to kits for thedetect-on and typing of the HSV in a sample. In addition, theinvention provides the nucleic acid and amino acid sequence of a subsequenceof HSV-2 Bgl II N having transforming activity. Further, theinvention teaches diagnostic and therapeutic methods for genital cancercomprising the use of these sequences or ligands directed thereto.