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Serum albumin and its purification method in humans with thiol-specific antioxidant activity
Serum albumin and its purification method in humans with thiol-specific antioxidant activity
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机译:具有硫醇特异性抗氧化活性的人血清白蛋白及其纯化方法
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摘要
FIELD OF THE INVENTION The present invention relates to serum albumin, methods for purification thereof, and use of serum serum albumin as protein antioxidants or membrane lipid peroxidants in humans having thiol-specific antioxidant activity. The thiol-specific serum albumin of the present invention is applied to human plasma with 70% ammonium sulfate treatment, DEAE-cellulose chromatography, 70% ammonium sulfate treatment, Sephadex G-75 gel chromatography, 70% ammonium sulfate treatment, phenyl sepharose It is purified through a series of processes of CL-4B column chromatography, 70% ammonium sulfate treatment and Sephadex G-100 gel chromatography. Purified human serum albumin is a thiol-specific antioxidant protein that prevents protein inactivation and peroxidation of membrane lipids, and may be used as an antioxidant in vivo including human blood. It can also be used for external ointments and cosmetics.
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