Device and method for vertical slab gel casting and electrophoresis in a single enclosure

摘要

A device and method for vertical slab gel casting and electrophoresis in a single enclosure is provided. Air is utilized as a sealing medium to seal the bottom of a gel space for gel casting by constructing an air- tight connection between a sealable lower buffer chamber and a gel space via a junction. The air is automatically released when a buffer is introduce into the lower buffer chamber so that the bottom of the gel is in direct contact with the buffer without manually removing a solid sealing device from the bottom of the gel, which enables the gel to be directly used for electrophoresis without any movement of the gel space.