Use of the Single Cell Gel Electrophoresis for Detecting in Marine Environmental Monitoring The Study of the Single Cell Gel Electrophoresis Detecting DNA Damage in Marine Bivalves

摘要

The single cell gel electrophoresis has been developed one kind of fast,simple,sensitive,inexpensive and reliable technology which examining eukaryotic cell DNA damage on single cell level since the end of 1980s.In this paper,single-cell gel electrophoresis (SCGE) in terms of principle and development is introduced; The achievement and progress of studies about the application of genetic toxicology assays in marine environmental monitoring are summarized,and the significance of genotoxicity research on marine environmental monitoring is stated.This paper seems timely to review the use of the comet assay to assess genotoxicant effects in the marine aquatic environment.Further work is required before the single cell gel electrophoresis assay can be used as a standard bio-indicator in marine environments,including standardization of methods and measurements in order to provide the new and important method of synthesize evaluate and risk assessment to monitor the marine environment in our country.%单细胞凝胶电泳是20世纪80年代末发展起来的一种快速、简单、灵敏、可靠,检验真核细胞DNA损伤的技术.本文介绍了单细胞凝胶电泳技术的发展与原理,概述了此技术在海洋环境的遗传毒性检测领域所取得的成果及研究进展,最后指出应用单细胞凝胶电泳技术开展海洋环境遗传毒性检测成为海洋环境的综合评价指标及为风险评价体系提供新方法和重要的手段的可行性.