Seaweed lectin and a method of manufacturing the same

摘要

PURPOSE: To obtain a novel seaweed lectin having characteristic agglutination activity and a specific sugar binding specificity by extracting the lectin from algae of Geldium amansii genus. CONSTITUTION: A seaweed lectin is extracted from algae of Geldium amansii genus by dipping the algae in a water-based medium prepared by adding a phosphate buffer to a physiological saline for about 12-24 hours at a temperature of 4-25 deg.C. The extracted solution is subjected to affinity chromatography using a chitin column and the adsorption fraction is eluted with 50-100mM NH4 OH, separated, and refined. The obtained lectin shows strong agglutination activity against the Group O human, rabbit, and rat red blood cells, but the agglutination disappears or decreases when the red blood cells are treated with pronase. In addition, the lectin does not show any agglutination activity against Group A and B human and sheep red blood cells. Moreover, the lectin strongly labels N-acetylchitotetraose, fetuin, asialofetuin, etc., and has a specific sugar binding property.