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EVALUATION METHOD OF SAMPLES OF BIOLOGICAL MATERIALS ANALYZED IN VITRO FOR DETERMINING CHEMOTHERAPY OF HIV POSITIVE PATIENTS, BASED ON PHENOTYPE MEDICINAL SENSITIVITY OF HUMAN HIV STRAINS
EVALUATION METHOD OF SAMPLES OF BIOLOGICAL MATERIALS ANALYZED IN VITRO FOR DETERMINING CHEMOTHERAPY OF HIV POSITIVE PATIENTS, BASED ON PHENOTYPE MEDICINAL SENSITIVITY OF HUMAN HIV STRAINS
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机译:基于人类HIV毒株的表型药物敏感性体外分析测定HIV阳性患者化学疗法的生物样品的评估方法
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摘要
A method for managing HIV chemotherapy of HIV positive patients comprises: (a) transfecting an HIV infection-susceptible cell line with an HIV pol gene sequence obtained from a patient and a HIV-DNA construct from which the sequence has been deleted; (b) culturing the transfected cells so as to create a stock of chimeric viruses; (c) assessing the phenotypic sensitivity of the chimeric viruses to an inhibitor of the HIV pol gene-encoded enzyme and assigning a value; (d) constructing a data set comprising the value for chimeric virus sensitivity and the corresponding value for a chimeric wild-type strain of HIV; (e) repeating the sensitivity assessment for at least two further inhibitors and thereby constructing at least three such data sets; (f) representing the data sets in 2-D or 3-D graphical form such that the difference between the chimeric and wild-type sensitivities in each data set provides a visual measure of the resistance of the chimeric stock to treatment by the inhibitor; and (g) selecting the optimum inhibitor(s) on the basis of the graphical representation of the resistances.
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