METHOD FOR CONTROLLING OF CHEMOTHERAPY OF HIV-POSITIVE PATIENTS BASED ON PHENOTYPE SENSITIVITY TO MEDICAMENTS OF HUMAN HIV STRAINS

摘要

The method ensures phenotype information about individualHIV-infested patients on a large scale, economically andexpressly. It can be applied to all presently availablechemiotherapeutical regimen and can be equally applicable to anyfuture regimen of this type. The method includes transfection of acellular line susceptible to HIV infection by a sequence,preferably the one encoding RT and protease from po1 gene of HIVproduced from a patient, and by HIV-DNA construct from which thesequence has been deleted. The transection cells are cultivated sothat a reserve of chimerical viruses beestablished after whichtheir pehotype sensitivity to the inhibitor of the enzyme encodedby the po1 gene of HIV is evaluated and in addition a value isdetermined. Then a set of data is constructed consisting of thesensitivity value of the chimerical virus and the respective valuefor the chimerical wild-type HIV strain, the sensitivityevaluation is repeated for at least two more inhibitors and inthis way at least three such sets of data are constructed as awhole. The sets of data are presented in two - or threedimensional graphic form so that the differences between thesensitivities of the chimerical and the wild type for each set ofdata shall ensure visual measurement of the resistance of thechimerical reserve of treatment by the inhibitor in question. Thenthe optimal inhibitor(s) is (are) selected based on the graphicrepresentation of the resistance measured.29 claims