PROCESS FOR PREPARING INFECTIOUS CLONE BASED ON GENOME OF +RNA VIRUS, RECOMBINANT NUCLEIC ACID COMPRISING THEREOF, MODIFIED RNA VIRUS CONTAINING THIS ACID AND VACCINE IN WHICH SAID MODIFIED RNA VIRUS IS CONTAINED

摘要

The present invention comprises a method for generating an infectious clone based on the genome of a positive strand RNA virus that has a genome of at least about 15 kb. The invention further comprises a method for generating an infectious clone based on the genome of a +RNA virus, said method further comprising selecting infectious clones by transfecting a host cell with a recombinant nucleic acid whereby a host cell is substantially not susceptible to infection with said virus. The invention further provides modified RNA viruses and vaccines and diagnostic assays derived therefrom.