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Method for Co-Preparation of Lymphocytosis Inducing Factor (LPF), Filamentous Hemagglutinin (FHA) and At Least
Method for Co-Preparation of Lymphocytosis Inducing Factor (LPF), Filamentous Hemagglutinin (FHA) and At Least
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机译:共制备淋巴细胞增多症诱导因子(LPF),丝状血凝素(FHA)的方法
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摘要
A process is provided for the production of lymphocytosis promoting factor (LPF), filamentous haemagglutinin (FHA) and at least one fimbrial agglutinogen from a liquid culture of Bordetella pertussis, which comprises the steps of (a) separating the culture into cellular and supernatant fractions, (b) concentrating the supernatant fraction, (c) fractionating the concentrated supernatant fraction to isolate LPF and FHA containing fractions, and (d) isolating at least one fimbrial agglutinogen from the cellular fraction. A vaccine composition may be produced by mixing so-produced LPF, FHA and fimbrial agglutinogens produced.
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